FIGURE 2.

Properties of hChlR1 helicase activity. Helicase assays were carried out using conditions described under “Experimental Procedures.” All reactions contained 36 fmol of hChlR1 and 5 fmol of the 39mer-M13 substrate, unless specified. A, nucleotide requirement for hChlR1 helicase activity. Each reaction contained, where noted, a 1 mm concentration of the indicated nucleoside 5′-triphosphate. B, unwinding of the 18mer-M13 or 39mer-M13 helicase substrate (18- and 39-mer, respectively) in the presence of increasing levels of hChlR1 and 1 mm ATP or dATP. C, the influence of various cations on hChlR1 helicase activity.