FIGURE 4.

Helicase activity of hChlR1 with various DNA substrates. DNA helicase reactions were carried out as described under “Experimental Procedures” with increasing amounts of hChlR1 and 5 fmol of the indicated partial duplex DNA substrates. In all panels shown, the conditions used were as follows. Lane 1, substrate only; lane 2, boiled substrate; lanes 3–5, 1, 3, or 9 fmol of enzyme, respectively; lane 6, 9 fmol of enzyme but no ATP. A, hChlR1 helicase translocates in the 5′–3′ direction. B, displacement of nicked and gapped DNA substrates by hChlR1. The size of the single strand gap separating the two labeled oligonucleotide is noted above the panels. The sequences of oligonucleotides used to generate the substrates are noted, and their sequences are summarized in Table 1.