FIGURE 2.

GPI-anchored constructs of CPX-I, -II, -III, and -IV inhibit cell fusion. A, domain structure of GPI-anchored constructs of CPX-I, -II, -III, and -IV compared with the solved crystal structure of CPX-I bound to SNARE pin. B, effect of different CPX-GPI (CPX-I through -IV; Cpx1-Cpx4) constructs on cell fusion (blue bars) and efficiency of cell fusion recovery after the addition of PI-PLC in the absence of SYT-I (yellow bars) and in the presence of SYT-I and calcium (red bars). Experiments are the mean ± S.E. of three independent experiments. C, dose-dependent inhibition of the cell fusion reaction using different soluble CPXs, sCPX-I, sCPX-I-(26-83), and sCPX-III. Results are the mean ± S.E. of two independent experiments.