TABLE 2.
rqh1 null mutation reduces intragenic recombination at ade6 similarly for three different allele-pair combinations
| Recombination frequency (Ade+ per 106 viable spores)
|
Mean fold-reduction in rqh1Δ | ||
|---|---|---|---|
| ade6 markers | wt | rqh1Δ | |
| M375 × 52 | 180, 240 | 18, 25 | 10 |
| M26 × 52 | 1,800, 1,800 | 390, 250 | 6 |
| M26 × 3049 | 17,000, 16,000 | 3,000, 2,200 | 6 |
For each genotype and combination of markers two crosses were done, and the ade6+ recombinant frequency measured. Crosses were: (top row, left to right) GP6318 × GP14, GP6 × GP13, GP6611 × GP6612, GP6610 × GP6613; (middle row, left to right) GP23 × GP14, GP24 × GP13, GP6609 × GP6612, GP6608 × GP6613; (bottom row, left to right) GP23 × GP4297, GP24 × GP4298, GP6609 × GP6614, GP6608 × GP6615. Additional ade6-M26 × ade6-52 data are shown in Figure 1B.