Figure 4.—

Deletion of DOT1 does not suppress the sensitivity of the rad52 and yku80 mutants to a DSB generated by the HO endonuclease at the MAT locus (A) Fivefold serial dilutions of log-phase cells growing in YPDA were spotted onto YPDA or YP–galactose plates (bottom, left and middle, respectively). Fivefold serial dilutions of log-phase cells incubated in liquid YP–galactose for 4 hr were spotted onto a YPDA plate (bottom right). Strains are YP943 (wild type), YP944 (dot1), YP945 (rad52), and YP946 (rad52 dot1). (B) Viability of JKM179-derived strains lacking HMLα and HMRa after incubation in YP–galactose for 3 hr to induce HO expression. Strains are JKM179 (wild type), YP815 (dot1), YP1167 (yku80), and YP1168 (yku80 dot1). Average and standard deviation values of three independent experiments are shown.