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. 2008 Jul;179(3):1197–1210. doi: 10.1534/genetics.108.089003

Figure 6.—

Figure 6.—

MMS-induced Rad52 foci formation and histone H2AS129 phosphorylation are increased in the dot1 mutant. (A) Rad52-YFP foci in wild-type and dot1 cells treated with 0.02% MMS for 1 hr. Representative fields are shown. Images are the maximum intensity projection from z-stacks of eight sections separated by 0.4 μm. The percentage of cells containing Rad52 foci (B) and the number of Rad52 foci per cell (C) are represented. (D) Western blot analysis of histone H2A phosphorylation at serine 129 in wild-type and dot1 cells treated with 0.02% MMS. Phosphoglycerate kinase (PGK) was used as a loading control. Strains are W3749-14C (wild type) and YP741 (dot1).