TABLE 1.
Yeast strains
| Strain | Genotype |
|---|---|
| BR1919αa | MATα leu2-3,112 his4-260 ura3-1 ade2-1 thr1-4 trp1-289 |
| BR1919a | MATaleu2-3,112 his4-260 ura3-1 ade2-1 thr1-4 trp1-289 |
| YP163 | BR1919α dot1∷TRP1 |
| YP210 | BR1919α PCH2-HA |
| YP345 | BR1919α dot1∷URA3 PCH2-HA |
| YP370 | BR1919α rad52∷LEU2 PCH2-HA |
| YP371 | BR1919α rad52∷LEU2 dot1∷TRP1 PCH2-HA |
| YP372 | BR1919α rad52∷LEU2 sir2∷URA3 PCH2-HA |
| YP513 | BR1919a yku80∷kanMX2 PCH2-HA |
| YP514 | BR1919a yku80∷kanMX2 dot1∷URA3 PCH2-HA |
| YP515 | BR1919a yku80∷kanMX2 rad52∷LEU2 PCH2-HA |
| YP516 | BR1919a yku80∷kanMX2 rad52∷LEU2 dot1∷URA3 PCH2-HA |
| YP544 | BR1919(a or α) sir3∷TRP1 |
| YP545 | BR1919a yku80∷kanMX2 sir3∷TRP1 |
| YP546 | BR1919a yku80∷kanMX2 sir3∷TRP1 dot1∷URA3 |
| YP547 | BR1919a sir3∷TRP1 dot1∷URA3 |
| YP548 | BR1919α rad52∷LEU2 sir3∷TRP1 |
| YP549 | BR1919α rad52∷LEU2 sir3∷TRP1 dot1∷URA3 |
| YP550 | BR1919a rad52∷LEU2 yku80∷kanMX2 sir3∷TRP1 |
| YP551 | BR1919α rad52∷LEU2 yku80∷kanMX2 sir3∷TRP1 dot1∷URA3 |
| YP574 | BR1919α rad54∷LEU2 PCH2-HA |
| YP576 | BR1919a dot1∷TRP1 |
| YP587 | BR1919α rad54∷LEU2 dot1∷URA3 PCH2-HA |
| YP588 | BR1919a rad54∷LEU2 yku80∷kanMX2 PCH2-HA |
| YP589 | BR1919a rad54∷LEU2 yku80∷kanMX2 dot1∷URA3 PCH2-HA |
| YP978 | BR1919a rad1∷kanMX4 |
| YP979 | BR1919a rad14∷hphMX4 |
| YP980 | BR1919a apn1∷kanMX4 |
| YP981 | BR1919a rad1∷kanMX4 dot1∷URA3 |
| YP983 | BR1919a rad1∷kanMX4 rad52∷LEU2 |
| YP985 | BR1919a rad1∷kanMX4 rad52∷LEU2 dot1∷URA3 |
| YP987 | BR1919a rad14∷hphMX4 dot1∷URA3 |
| YP989 | BR1919a rad14∷hphMX4 rad52∷LEU2 |
| YP990 | BR1919a rad14∷hphMX4 rad52∷LEU2 dot1∷URA3 |
| YP992 | BR1919a apn1∷kanMX4 dot1∷URA3 |
| YP994 | BR1919a apn1∷kanMX4 rad52∷LEU2 |
| YP995 | BR1919a apn1∷kanMX4 rad52∷LEU2 dot1∷URA3 |
| W303-1A | MATaleu2-3,112 trp1-1 ura3-1 ade2-1 his3-11,15 can1-100 rad5-G535R |
| W303-1B | MATα leu2-3,112 trp1-1 ura3-1 ade2-1 his3-11,15 can1-100 rad5-G535R |
| YP435 | W303-1A zip1∷LEU2 PCH2-HA |
| YP436 | W303-1A PCH2-HA |
| YP439 | W303-1A yku80∷kanMX2 zip1∷LEU2 PCH2-HA |
| YP452 | W303-1A dot1∷URA3 zip1∷LEU2 PCH2-HA |
| YP454 | W303-1A yku80∷kanMX2 dot1∷URA3 zip1∷LEU2 PCH2-HA |
| YP506 | W303-1A dot1∷URA3 PCH2-HA |
| YP520b | W303-1A rad9∷HIS3 |
| YP521b | W303-1A rad24∷TRP1 |
| YP522b | W303-1A rad9∷HIS3 rad24∷TRP1 |
| YP523b | W303-1A rad9∷HIS3 dot1∷URA3 |
| YP524b | W303-1B rad24∷TRP1 dot1∷URA3 |
| YP525b | W303-1A rad9∷HIS3 rad24∷TRP1 dot1∷URA3 |
| W3749-14Cc | W303-1A RAD5 ADE2 bar1∷LEU2 RAD52∷YFP |
| W3483-10Ac | W303-1A RAD5 ADE2 bar1∷LEU2 MRE11∷YFP |
| YP741 | W303-1A RAD5 ADE2 bar1∷LEU2 RAD52∷YFP dot1∷TRP1 |
| YP756 | W303-1A RAD5 ADE2 bar1∷LEU2 MRE11∷YFP dot1∷kanMX6 |
| YP943 | W303-1A leu2∷SFA1 ade3∷GAL∷HO |
| YP944 | W303-1A leu2∷SFA1 ade3∷GAL∷HO dot1∷TRP1 |
| YP945 | W303-1A ade3∷GAL∷HO rad52∷LEU2 |
| YP946 | W303-1A ade3∷GAL∷HO rad52∷LEU2 dot1∷TRP1 |
| BY4741 | MATahis3Δ0 leu2Δ0 ura3Δ0 met15Δ0 |
| BY4742 | MATα his3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 |
| BY4741-dot1Δ | BY4741 dot1∷kanMX4 |
| BY4741-rad30Δ | BY4741 rad30∷kanMX4 |
| BY4741-rev1Δ | BY4741 rev1∷kanMX4 |
| BY4741-rev3Δ | BY4741 rev3∷kanMX4 |
| YP811 | BY4741 rad52∷LEU2 |
| YP1080 | BY4741 rad30∷kanMX4 dot1∷URA3 |
| YP1081 | BY4741 rev1∷kanMX4 dot1∷URA3 |
| YP1082 | BY4741 rev3∷kanMX4 dot1∷URA3 |
| YP1125 | BY4741 rev3∷kanMX4 rad52∷LEU2 |
| YP1126 | BY4741 rev3∷kanMX4 rad52∷LEU2 dot1∷URA3 |
| YP1196 | BY4741 rev1∷kanMX4 rad52∷LEU2 |
| YP1197 | BY4741 rev1∷kanMX4 rad52∷LEU2 dot1∷URA3 |
| JKM179d | MATα hml∷ADE1 hmr∷ADE1 ade1-110 leu2, 3-112 lys5 trp1∷hisG ura3-52 ade3∷GAL10∷HO |
| YP815 | JKM179 dot1∷TRP1 |
| YP1167 | JKM179 yku80∷kanMX2 |
| YP1168 | JKM179 yku80∷kanMX2 dot1∷TRP1 |
a
BR1919α was provided by Shirleen Roeder (Yale University) and corresponds to BR1919-8B (Rockmill and Roeder 1990). BR1919a was generated by switching the mating type of BR1919α.
b
These strains are segregants from the cross YP506 × DLY262. The strain DLY262 (W303-1B rad9∷HIS3 rad24∷TRP1) was provided by Ted Weinert (University of Arizona). The presence of PCH2-HA was not followed in these segregants.
c
These strains, provided by Rodney Rothstein, have been described (Lisby et al. 2003, 2004).
d
Provided by Jim Haber (Lee et al. 1998).