TABLE 2.

Plasmids used in this study and the source or construction

Name	Relevant information
pCW4	pACYC184 derivative encoding TnsABCDE, tetracycline resistant (Waddell and Craig 1988).
pCW15	pACYC184 derivative encoding TnsABC, chloramphenicol resistant (Waddell and Craig 1988).
pCW15*	pACYC184 derivative encoding TnsABCA225V, chloramphenicol resistant (Stellwagen and Craig 1997b).
pJP123	pACYC184 derivative encoding TnsABC+E, chloramphenicol resistant (Peters and Craig 2000).
pTA106	pSC101 replicon, ampicillin-resistant cloning vector (Peters and Craig 2000).
pJP104	pTA106 derivative encoding TnsE, ampicillin resistant (Peters and Craig 2000).
pQS100	pTA106 vector encoding TnsABC constructed by cloning the 4919 bp of PvuII fragment encoding TnsABC from pCW4 into the SmaI site of pTA106 with the tnsC gene proximal to the vector HindIII site.
pQS101	pTA106 vector encoding TnsABC constructed by cloning the 4919 bp of PvuII fragment encoding TnsABC from pCW4 into the SmaI site of pTA106 with the tnsA gene proximal to the vector HindIII site.
pQS102	pTA106 vector encoding TnsABC+E constructed by cloning the 4919 bp of PvuII fragment encoding TnsABC from pCW4 into the SmaI site of pJP104 vector with the tnsC gene proximal to the vector HindIII site.
pQS103	pTA106 vector encoding TnsABC+E constructed by cloning the 4919 bp PvuII fragment encoding TnsABC from pCW4 into the SmaI site of pJP104 vector with the tnsA gene proximal to the vector HindIII site.
pQS107	pTA106 vector encoding TnsABCA225V constructed by cloning a 726 bp KpnI-StuI fragment encoding the Alanine(225) to Valine amino acid change of tnsC from pCW15* into pQS101.
pZT383	pMB1 vector with lacIq regulation expressing Tn10 transposase mutant P167S, ampicillin resistant (Galitski and Roth 1997).
pJP135	pMB1 vector with lacIq regulation expressing Tn10 transposase mutant D97A/D161A, ampicillin resistant (Peters and Craig 2000).
pCP20	Temperature-sensitive plasmid with thermal induction of FLP recombinase (Cherepanov and Wackernagel 1995).
pKD46	Temperature-sensitive plasmid with the λ Red proteins under arabinose control (Datsenko and Wanner 2000).
pKD3	Plasmid encoding ampicillin resistance that allows PCR amplification of a gene cassette encoding chloramphenicol resistance flanked by frt sites recognized by the FLP recombinase (Datsenko and Wanner 2000).