TABLE 2.
Phenotypic characteristics of N. crassa MAPK pathway single and respective cot-1;MAPK double mutants
| Growth ratesa
|
Vegetative fusion
|
Major hyphal defects of MAP kinase mutantsb | Female fertilityc
|
|||||
|---|---|---|---|---|---|---|---|---|
| Strain | mapk | cot-1(ts);mapk | mapk | cot-1(ts);mapk | Asexual development of MAP kinase mutantsb | mapk | cot-1(ts);mapk | |
| Wild type | 3.5 | Yes | Yes | |||||
| cot-1(ts) | 3.2 | Yes | Yes | |||||
| Osmosensing pathway | ||||||||
| Δos-4 (NCU03071) | 3.2 | NDd | No | NDd | ± Wild type; frequent tip splitting | Conidial lysis | No | NDd |
| Δos-5 (NCU00587) | 3.1 | 3.0 | No | No | No | No | ||
| Δos-2 (NCU07024) | 3.2 | 3.2 | No | No | No | No | ||
| Cell fusion/fertility pathway | ||||||||
| Δnrc-1 (NCU06182) | 1.2 | 2.5 | No | Yes | Highly irregular growth axisb | Reduced aerial hyphae and conidia formationb | No | Yes |
| Δmek-2 (NCU04612) | 1.1 | NDd | No | NDd | No | NDd | ||
| Δmak-2 (NCU02393) | 1.2 | 2.6 | No | Yes | No | Yes | ||
| Cell-wall integrity pathway | ||||||||
| Δmik-1 (NCU02234) | 0.6 | NDd | No | NDd | Polarity defect; branch formation abnormal | Arthroconidiation for mak-1 and mek-1 | No | NDd |
| Δmek-1 (NCU06419) | 0.5 | 0.6 | No | No | No | No | ||
| Δmak-1 (NCU11376) | 0.6 | 0.5 | No | No | No | No | ||
In centimeters/day at 20° (n = 3) as determined by radial hyphal growth experiments.
No differences in hyphal morphology and asexual development were observed for the single and respective cot-1 double mutants grown at 25° except for a better condidation rate of the cot-1;mak-2 and cot-1;nrc-1 double mutants compared to mak-2 and nrc-1.
Protoperithecia formation after 7 days at room temperature on 2% cornmeal agar supplemented with 0.1% glucose and viable ascospore formation when fertilized with wild-type conidia.
Not determined, as we were not able to obtain viable hygromycin-resistant ascospores in crosses with wild type or cot-1 as the female partner.