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Backcross design for QTL mapping population. Queen P2 and worker P1 were reared from the anarchy line. Drone P3 was collected from a wild-type colony that (typically) did not show any signs of worker-laid brood. A presumably heterozygous F1 queen F12 was mated to an F1 anarchist drone F11 to give the F2 generation of workers. The F2 generation was used as the mapping population.
