Figure 1.—

Mutations in the dRYBP gene, 5′-RACE and quantitative PCR analysis. (A) The dRYBP gene structure, showing the positions of the two in-frame ATGs codons. dRYBP¹ contains the P[KG08683]-element insertion (indicated as a triangle) at nucleotide position 322. dRYBP^Δ16 is an incomplete deletion of the P[KG08683] element. dRYBP^Δ55 deletes nucleotides 322–853. (B) The dRYBP protein contains an NZF domain (red) located in the amino terminus. The putative dRYBP^Δ55 protein encoded by dRYBP^Δ55 does not contain the NZF domain (red). (C) 5′-RACE analysis was performed using total RNA isolated from homozygous Df(1)^w67c23 (yw) and homozygous dRYBP^Δ55 embryos. The reverse-transcription (RT) reaction was performed in the presence and in the absence of Tobacco acid pyrophosphatase (Tap). A control containing “minus-Tap”-treated RNA was also performed. A band of 560 bp (arrow) was observed, corresponding to the dRYBP wt 5′-RACE product (lane yw, “plus-RT/plus-Tap”; the smaller band in this lane is an artifact). A band of 380 bp (arrowhead) corresponds to the dRYBP^Δ55 5′-RACE product (lane dRYBP^Δ55, plus-RT/plus-Tap). The band in the second lane of dRYBP^Δ55 (marked with *) corresponds to “plus-RT/minus-Tap” is an artifact of the 5′-RACE protocol used (Instructions manual, FirstChoice-RLM-RACE; Ambion). (D) Quantitative PCR results showing the relative mRNA expression levels from homozygous Df(1)^w67c23 (yw), dRYBP¹, and dRYBP^Δ16 early first-instar larvae and adults.