Figure 2.—

Nuclear localization pattern of dRYBP protein. (A) dRYBP protein localization (green) in the embryonic nuclei. dRYBP nuclear bodies appear as both large (arrows) and small (arrowheads) structures. (B) H3K27me3 (red) colocalizes (circle) with dRYBP in some of the nuclear bodies (circle). (C) Merged image of A and B. The circles indicate colocalization in some of the bodies. (D) Staining of DAPI (blue) and dRYBP (green). (E) Embryonic nuclear distribution of dRYBP (red). (F) GFP nuclear localization of PC-EGFP transgenic embryos (circle indicates colocalization). (G) Merged image of E and F. (H) Daunomycine staining [used to label the nucleoli (red, arrowhead)] and dRYBP protein expression (blue, arrow) do not colocalize in the nuclei of the wing imaginal disc cells. (I–L) Localization of dRYBP protein (I, red), To-PRO (J, blue), and α-tubulin (K, green) during metaphase of syncytial embryonic nuclear divisions. dRYBP does not localize with To-PRO (J). (L) Merged image of I–K. (M–P) Localization of dRYBP protein (M, red), To-PRO (N, blue), and α-tubulin (O, green) during anaphase of syncytial embryonic nuclear divisions. dRYBP (red) colocalizes with To-PRO (blue). (P) Merged image of M–O.