Table 2.

Antibodies

Epitope	Cryo/paraffin	Antigen retrieval	Primary antibody, dilution, and source	Secondary antibody	Reference
α3 integrin	Cryo	None	Rabbit anti-α3 integrin, 1:800, Chemicon cat# AB1920	Anti-rabbit Envision*
α-actinin-4	Cryo	None	Rabbit polyclonal antibody 6A3, 1:4000	Anti-rabbit Envision	8
α-dystroglycan	Paraffin	Citrate†	Mouse mAb VIA4-1, 1:150, Upstate cat# 05-298	Anti-mouse Envision
Desmin	Paraffin/cryo	Tris/EDTA‡	Mouse mAb clone 33, 1:750	Anti-mouse Envision, Alexa
Ezrin	Paraffin	Tris/EDTA	Mouse mAb, 1:100	Anti-mouse Envision
Nephrin (extra-cellular domain)	Cryo	None	Mouse mAb 5-1-6, 1:200	Anti-mouse Envision	51
Podocalyxin	Paraffin	Proteinase K§	Mouse mAb 5A, 1:300	Anti-mouse Envision	52
Podocin (C-terminal part)	Paraffin	Proteinase K	Rabbit polyclonal antibody P35, 1:3000	Anti-rabbit Envision	53
Podoplanin	Paraffin/cryo	Proteinase K	Rabbit polyclonal anti-rat podoplanin, 1:3000	Anti-rabbit Envision, Alexa	54
Synaptopodin	Paraffin	Citrate	Mouse mAb G1D4, 1:4, Progen cat# 65194	Anti-mouse Envision
Wilms tumor 1	Paraffin	Tris/EDTA	Mouse mAb WLM04, 1:50, Abcam cat# ab3236	Anti-mouse Envision

^*Envision: DakoCytomation, Glostrup, Denmark, Cat# K4001 (anti-mouse Envision HRP) and K4003 (anti-rabbit Envision HRP). 

^†Citrate: 0.1 mol/L citrate buffer, pH 6.0. 

^‡Tris/EDTA: 0.1 mol/L Tris/EDTA, pH 9.0 

^§Proteinase K: DakoCytomation, Glostrup Denmark, Cat# S3020.