Table 2.
Epitope | Cryo/paraffin | Antigen retrieval | Primary antibody, dilution, and source | Secondary antibody | Reference |
---|---|---|---|---|---|
α3 integrin | Cryo | None | Rabbit anti-α3 integrin, 1:800, Chemicon cat# AB1920 | Anti-rabbit Envision* | |
α-actinin-4 | Cryo | None | Rabbit polyclonal antibody 6A3, 1:4000 | Anti-rabbit Envision | 8 |
α-dystroglycan | Paraffin | Citrate† | Mouse mAb VIA4-1, 1:150, Upstate cat# 05-298 | Anti-mouse Envision | |
Desmin | Paraffin/cryo | Tris/EDTA‡ | Mouse mAb clone 33, 1:750 | Anti-mouse Envision, Alexa | |
Ezrin | Paraffin | Tris/EDTA | Mouse mAb, 1:100 | Anti-mouse Envision | |
Nephrin (extra-cellular domain) | Cryo | None | Mouse mAb 5-1-6, 1:200 | Anti-mouse Envision | 51 |
Podocalyxin | Paraffin | Proteinase K§ | Mouse mAb 5A, 1:300 | Anti-mouse Envision | 52 |
Podocin (C-terminal part) | Paraffin | Proteinase K | Rabbit polyclonal antibody P35, 1:3000 | Anti-rabbit Envision | 53 |
Podoplanin | Paraffin/cryo | Proteinase K | Rabbit polyclonal anti-rat podoplanin, 1:3000 | Anti-rabbit Envision, Alexa | 54 |
Synaptopodin | Paraffin | Citrate | Mouse mAb G1D4, 1:4, Progen cat# 65194 | Anti-mouse Envision | |
Wilms tumor 1 | Paraffin | Tris/EDTA | Mouse mAb WLM04, 1:50, Abcam cat# ab3236 | Anti-mouse Envision |
Envision: DakoCytomation, Glostrup, Denmark, Cat# K4001 (anti-mouse Envision HRP) and K4003 (anti-rabbit Envision HRP).
Citrate: 0.1 mol/L citrate buffer, pH 6.0.
Tris/EDTA: 0.1 mol/L Tris/EDTA, pH 9.0
Proteinase K: DakoCytomation, Glostrup Denmark, Cat# S3020.