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CCAAT enhancer binding site is critical for PGE2-induced StAR promoter activity. Cells were transfected with reporter constructs harboring human StAR promoter (−327 StAR-luc) with site-directed mutations at specific transcription factor binding sites as indicated. Data show means and standard errors of three independent experiments using different batches of cells. *Significant difference between control and PGE2-treated groups at P < 0.05.
