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. 2008 Aug;173(2):463–469. doi: 10.2353/ajpath.2008.071044

Figure 3.

Figure 3

A: Proliferative effect of IL-4 on ESCs. ESCs were treated with IL-4 at different concentrations for 72 hours. The proliferation of ESCs was evaluated by cell counting kit-8 (CCK-8). Values are the mean ± SEM of combined data from five independent experiments using different ESC preparations from different women. *P < 0.01; **P < 0.0001 (each versus control). B: Effect of IL-4 on BrdU incorporation in ESCs. ESCs were treated with IL-4 at different concentrations for 48 hours, and then BrdU incorporation into DNA was measured. Values are the mean ± SEM of combined data from four independent experiments using different ESC preparations from different women. *P < 0.01; **P < 0.0001 (each versus control). C: Effect of anti-IL-4 receptor neutralizing antibody on IL-4-induced proliferation of ESCs. After the preincubation with either anti-IL-4 receptor neutralizing antibody or isotype IgG2a for 30 minutes, the cells were treated with or without IL-4 for 72 hours. Proliferation of ESCs was evaluated by CCK-8. Values are the mean ± SEM of the combined data from five independent experiments using different ESC preparations. *P < 0.0001 (versus isotype IgG); **P < 0.0001 (versus IL-4 plus isotype IgG).