Figure 4.

DLP1 knockdown by miR RNAi or dominant negative DLP1 mutant (ie, DLP1 K38A) overexpression causes abnormal mitochondria distribution in NHFs. A and B: A representative immunoblot (A) and quantification analysis (B) confirms significantly decreased levels of DLP1in DLP1 miR RNAi transfected cells compared to non-transfected NHFs (Cont.) or negative miR RNAi vector-transfected NHFs (negative Cont.) (*P < 0.05; Student’s t-test). Equal protein amounts (15 μg) were loaded. C: Quantitative analysis reveals significantly more positively transfected NHFs (ie, either DLP1 miR RNAi-transfected or DLP1 K38A-transfected) demonstrating an abnormal mitochondrial distribution pattern compared to vehicle-transfected NHFs (P < 0.05). Mitochondrial length was significantly increased (D) while number significantly decreased (E) even in transfected cells without apparent abnormal distribution.