Figure 6.

Hydrogen peroxide causes mitochondrial abnormalities and changes levels of DLP1 in NHFs and exacerbated that of sAD fibroblasts, and can be abolished by vitamin E pretreatment. A: ROS levels in NHFs and sAD fibroblasts (*P < 0.05 compared to vehicle without H₂O₂ treatment. B: Significantly more NHFs and sAD fibroblasts demonstrate an abnormal mitochondrial distribution pattern with mitochondrial clustering around the nucleus after hydrogen peroxide treatment, which was totally abolished by vitamin E pretreatment. WT DLP1 overexpression prevents hydrogen peroxide-induced mitochondrial network collapse. A representative immunoblot (C) and quantification analysis (D) demonstrates that hydrogen peroxide-induced DLP1 reduction in NHFs and further reduction in sAD fibroblasts is abrogated by vitamin E pretreatment.