Figure 2.

Expression pattern of DFak56. (A) Developmental Northern blot hybridized with a 5′ probe from DFak56 cDNA. Three bands are detected: 4.7, 5.3, and 6.5 kb. The times refer to hours of embryonic development. IL, instar larva; EP and LP, early and late pupa, respectively. The reduction in expression in larval and pupal stages is exaggerated because of loading differences [as determined by a probe to RP49 (not shown)]. (B–F). Staged embryos stained with a DFak56 anti-sense RNA probe. Representative examples of stage 3 (B), 5 (C), 11 (D), 13 (E), and 14 (F) embryos are shown; br, brain; ms, mesoderm. (G) A stage 13 embryo stained with a DFak56 sense RNA probe.