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Examination of ES cell nuclei 4 h after transfer into enucleated oocytes, without activation by Sr2+
| ES cells* | No. of oocytes examined | No. (%) of oocytes
with
|
|||
|---|---|---|---|---|---|
| Intact nucleus | Chromosomes | Indistinct chromatin condensation | Pseudo-pronuclei (spontaneous activation) | ||
| Small | 75 | 4 (5.3) | 38 (50.7) | 32 (42.7) | 1 (1.3) |
| Large | 59 | 4 (6.8) | 40 (67.8) | 11 (18.6) | 4 (6.8) |
*Average cell diameters are 10 μm (small) and 18 μm (large).
