Table 2.
ES cells* | No. of oocytes examined | No. (%) of incoming nuclei transformed to one
or more pseudo-pronuclei (pp) with or without extrusion of a
pseudo-second polar body (ppb)
|
||||
---|---|---|---|---|---|---|
Not transformed | 1pp and ppb | 1pp | 2pp | 3pp | ||
Small | 23 | 0 | 0 | 1 (4.3) | 18 (78.3) | 4 (17.4) |
Large | 22 | 1 (4.5) | 15 (68.2) | 3 (13.6) | 3 (13.6) | 0 |
Reconstructed oocytes were activated in the presence (small cells) or absence (large cells) of cytochalasin B as described in Materials and Methods. Cell sizes are as for Table 1.