Table 3.
Development of embryos from enucleated oocytes injected with E14 ES cell nuclei
| ES cells* | Conc. of FCS (%) in culture medium | No. of enucleated oocytes injected | No. (%) of activated oocytes | No. (%) of morulae/blastocysts developed | No. of transferred embryos (recipients) | Postimplantation development
|
|||
|---|---|---|---|---|---|---|---|---|---|
| Total no. (%) of implantation sites | No. (%) of placentae alone | No. (%) of dead fetuses† | No. (%) of live offspring | ||||||
| Large | 15 | 87 | 72 (82.8) | 32 (44.4) | 32 (3) | 17 (53.1) | 0 | 0 | 0 |
| 5 | 152 | 135 (88.8) | 58 (43.0) | 51 (5) | 31 (60.8) | 1 (2.0) | 2 (3.9) | 1 (2.0) | |
| 3–4 | 117 | 91 (77.8) | 22 (24.2) | 22 (3) | 16 (72.7) | 3 (13.6) | 0 | 0 | |
| 1–2 | 93 | 79 (84.9) | 27 (34.2) | 27 (3) | 24 (88.9) | 0 | 1 (3.7) | 0 | |
| 0.5–0.75 | 16 | 9 (56.3) | 3 (33.3) | 3 (1) | 2 (66.7) | 0 | 0 | 0 | |
| Subtotal | 465 | 386 (83.0) | 142 (36.8) | 135 (15) | 90 (66.7) | 4 (3.0) | 3 (2.2) | 1 (0.7) | |
| Small | 15 | 48 | 38 (79.2) | 12 (31.6) | 12 (2) | 0 | 0 | 0 | 0 |
| 5 | 566 | 466 (82.3) | 65 (13.9) | 63 (7) | 40 (63.5) | 5 (7.9) | 3 (4.8) | 2 (3.2) | |
| 3–4 | 154 | 141 (91.6) | 32 (22.7) | 32 (3) | 18 (56.3) | 2 (6.3) | 1 (3.1) | 2 (6.3) | |
| 1–2 | 388 | 326 (84.0) | 55 (16.9) | 55 (7) | 35 (63.6) | 4 (7.3) | 1 (1.8) | 0 | |
| 0.5–0.75 | 144 | 126 (87.5) | 17 (13.5) | 17 (2) | 17 (100) | 0 | 0 | 0 | |
| Subtotal | 1,300 | 1,097 (84.4) | 181 (16.5) | 179 (21) | 110 (61.5) | 11 (6.1) | 5 (2.8) | 4 (2.2) | |
| Total | 1,765 | 1,483 (84.0) | 323 (21.8) | 314 (36) | 200 (63.7) | 15 (4.8) | 8 (2.5) | 5 (1.6) | |
*Cell sizes are as for Table 1.
†Estimated to have died at 15–17 dpa.