Table 4.
Development of embryos from enucleated oocytes injected with R1 ES cell nuclei
| ES cells* | Conc. of FCS (%) in culture medium | No. of enucleated oocytes injected | No. (%) of activated oocytes | No. (%) of morulae/blastocysts developed | No. of transferred embryos (recipients) | Postimplantation development
|
|||
|---|---|---|---|---|---|---|---|---|---|
| Total no. (%) of implantation sites | No. (%) of placentae alone | No. (%) of dead fetuses† | No. (%) of live offspring | ||||||
| Large‡ | 5 | 197 | 153 (77.7) | 47 (23.9) | 47 (6) | 36 (76.6) | 0 | 2 (4.3) | 3 (6.4) |
| (Nocod.) | |||||||||
| Small | 15§ | 364 | 317 (87.1) | 129 (35.4) | 129 (11) | 78 (60.5) | 2 (1.6) | 5 (3.9) | 7 (5.4) |
| 5 | 275 | 212 (77.1) | 73 (26.5) | 73 (9) | 59 (80.8) | 5 (6.8) | 9 (12.3) | 9 (12.3) | |
| 0.5§ | 251 | 193 (76.9) | 63 (25.1) | 63 (7) | 44 (69.8) | 4 (6.3) | 4 (6.3) | 7 (11.1) | |
| Total | 1,087 | 875 (80.5) | 312 (28.7) | 312 (33) | 217 (69.6) | 11 (3.5) | 20 (6.4) | 26 (8.3) | |
*Cell sizes are as for Table 1.
†Estimated to have died at 13–17 dpa.
‡Nocodazole treatment.
§ Three recipient females delivered naturally. The uteri of these females could not be observed, such that the number of implantation sites, placentae, and dead fetuses are not included these females' data.