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. 1999 Dec 21;96(26):14990–14995. doi: 10.1073/pnas.96.26.14990

Figure 2.

Figure 2

Two distinct forms of C. elegans Mediator complexes. (A) Immunoprecipitation of C. elegans embryo nuclear extracts with the Mediator Abs indicated at the top of each lane. Equivalent amount of nuclear extract (N), supernatant (S), and pellet (P) of the immunoprecipitation were resolved on SDS/PAGE and immunoblotted with the Abs indicated at the left. (B) The heparin-Sepharose column fractions of C. elegans nuclear extract (N) eluted with the potassium acetate salt concentration (molar) indicated at the top of each lane were analyzed by SDS/PAGE and immunoblotting with the Abs indicated at the left. (C) CeMed6 and CeSrb7 complexes. Heparin fractions containing CeMed6 (H3) and CeSrb7 (H6) were immunoprecipitated with CeMed6 and CeSrb7 Abs, respectively, and the proteins that coprecipitated with CeMed6 or CeSrb7 were visualized by SDS/PAGE and immunoblot analysis with the Abs indicated at the left. (D) Superose-6 gel-filtration chromatography of C. elegans Mediator complexes. C. elegans nuclear extracts were fractionated on a Superose-6 column, and the fractions were subjected to SDS/PAGE and immunoblotted with the Abs indicated at the left. The migration positions of molecular size markers are indicated (kDa).