Figure 4.

Embryonic lethality caused by RNAi of CeMED6. This series of photographs (A– H) shows embryos collected from animals injected with CeMED6 dsRNA at 0, 1, 3, 6, 8, 11, 14, and 33 h after transferring the injected animals to a slide glass. Embryo is shown at four-cell stage (A), 20-cell stage (B), ≈60-cell stage (C), and ≈300-cell stage (D). There are no visible abnormalities in the embryo up to this stage. However, these embryos never developed to the comma stage (E and F). (G) The pharynx and the gut granules were formed, and their movements were detected. (H) Internal cells of the embryo began to disintegrate, and no movement was observed. These results are representative of those observed in RNAi analysis of CeMED7 and CeMED10/CeNUT2.