Figure 1.

In situ hybridization, showing expression of Lhx8 mRNA at different stages of palatal development. (A) Expression of Lhx8 in the maxillary processes (arrowhead) of an E11.5 embryo. (B) Expression of Lhx8 in the palatal shelves (arrowhead) of an E13.5 embryo, before shelf elevation. (C) Expression of Lhx8 in the palate shelves (arrowheads) of an E14.5 embryo, after shelf elevation and contact. (D) Continuous expression of Lhx8 in the palate of an E16.5 embryo, after fusion of the palate shelves. Notice that Lhx8 was also expressed in the tooth bud (arrows), tongue (t), and the ventral forebrain (b). Scale bar in A represents 200 μm for all panels.