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Scheme of the differential hybridization technique with arrayed cDNA. Amplified cDNAs were arrayed on a nylon membrane to make a high-density filter. The filter was then hybridized with probes prepared from wild-type and mutant worms. Hybridized signals were detected by using the Fuji BAS system and quantified with hdg analyzer software (Genomic Solutions). Comparing the intensities between wild-type and mutant hybridizations identified differentially expressed genes.
