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. 1999 Dec 21;96(26):15026–15031. doi: 10.1073/pnas.96.26.15026

Figure 2.

Figure 2

Determination of the size and organization of the MAT locus of U. hordei by hybridization with probes from the b gene complex. (A) Ethidium-bromide-stained CHEF gel. (B–D) DNA gel blots of the same gel hybridized with the probes indicated. Lane 1, I-SceI-digested 4857–4 (a1b1); lane 2, I-SceI-digested 364–86 (a1b1; single tag at a1); lane 3, undigested 364–86dt21 (a1b1; double tag at a1, b1); lane 4, I-SceI-digested 364–86dt21 (a1b1; double tag at a1, b1); lane 5, I-SceI-digested 365–57dt51 (a2b2; double tag at a2, b2); lane 6, undigested 365–57dt51 (a2b2; double tag at a2, b2); lane 7, I-SceI-digested 365–57 (a2b2; single tag at b2); lane 8, I-SceI-digested 4857–5 (a2b2). Note that probes b1–1, be and bw recognize homologous sequences in both MAT loci.