Table 1.
Strain | Genotype* | Source |
---|---|---|
U. hordei 549 | a2b1 (Δb2, UhbWE1, hygr, phleor)† | (11) |
U. hordei 550 | a2b1 (Δb2, UhbWE1, hygr, phleor)† | (11) |
U. hordei 551 | a1b2 (Δb1, UhbW2, hygr, phleor)† | (11) |
U. hordei 552 | a1b2 (Δb1, UhbW2, hygr, phleor)† | (11) |
U. hordei 4857-4 | a1b1 (wild-type strain) | P. Thomas (11) |
U. hordei 4857-5 | a2b2 (wild-type strain) | P. Thomas (11) |
U. hordei 364-62/364-86 | a1b1 (Δpan1∷phleor/SceI)‡ | This work |
U. hordei 364-86dt21 | a1b1 (Δpan1∷phleor/SceI, ΔbWE1:hygr/SceI)§ | This work |
U. hordei 365-57/365-71 | a2b2 (ΔbW2∷hygr/SceI)¶ | This work |
U. hordei 365-57dt51 | a2b2 (ΔbW2∷hygr/SceI, Δpan1∷phleor/SceI)∥ | This work |
*phleor, phleomycin resistance; hygr, hygromycin B resistance; SceI, I-SceI recognition sequence.
† In these strains, the resident b genes were inactivated by insertion of a hygromycin B resistance marker; subsequently, functional b gene complexes (pUhbWE1 or pUhbW2) were integrated at ectopic sites (11).
‡These strains were constructed by transformation of strain 4857-4 with pSce-phleo#4 at a1.
§This strain was constructed by transformation of strain 364-86 with pSceI-Hyg#1 at b1.
¶These strains were constructed by transformation of strain 4857-5 with pSceI-Hyg#1 at b2.
∥This strain was constructed by transformation of strain 365-57 with pSce-phleo#4 at a2.