Skip to main content
. 1999 Dec 21;96(26):15026–15031. doi: 10.1073/pnas.96.26.15026

Table 2.

Segregation of markers in crosses to detect recombination within MAT

Cross Ratio of progeny with the following genotypes:
MAT-1/ MAT-2* phleor/ phleos hygs/ hygr phleor, hygr/ phleos, hygs
1 24/25 24/25 24/25 6/18,400
2 23/26 23/26 23/26 7/18,400
3 26/24 26/24 26/24 10/17,800
4 35/15 35/15 35/15 11/17,400
Total 108/90 108/90 108/90 34/72,000
*

The genotypes of the progeny were determined by performing mating tests with wild-type strains 4857-4 and 4857-5. 

Two hundred random progeny were tested for resistance to phleomycin (phleor) or hygromycin B (hygr). 

For each cross, 20, 000 progeny were inoculated onto culture medium. An average viability of ∼90% was obtained for each cross. A total of 72,000 viable progeny were tested for resistance to both antibiotics. A second trial identified 36 progeny (out of 70,420) that were resistant to both antibiotics.