Table 2.
Cross | Ratio of progeny with the following genotypes:
|
|||
---|---|---|---|---|
MAT-1/ MAT-2* | phleor/ phleos† | hygs/ hygr† | phleor, hygr/ phleos, hygs‡ | |
1 | 24/25 | 24/25 | 24/25 | 6/18,400 |
2 | 23/26 | 23/26 | 23/26 | 7/18,400 |
3 | 26/24 | 26/24 | 26/24 | 10/17,800 |
4 | 35/15 | 35/15 | 35/15 | 11/17,400 |
Total | 108/90 | 108/90 | 108/90 | 34/72,000 |
The genotypes of the progeny were determined by performing mating tests with wild-type strains 4857-4 and 4857-5.
† Two hundred random progeny were tested for resistance to phleomycin (phleor) or hygromycin B (hygr).
‡ For each cross, 20, 000 progeny were inoculated onto culture medium. An average viability of ∼90% was obtained for each cross. A total of 72,000 viable progeny were tested for resistance to both antibiotics. A second trial identified 36 progeny (out of 70,420) that were resistant to both antibiotics.