Table 1.

Relative delay in nucleotide excision repair for LF041 skin fibroblasts, and for lung fibroblasts expressing the HPV E6, HPV E7, or both oncoproteins

Fibroblast type	Strand	Average 50% repair time, hr*	Fold delay in repair	No. and location of sites analyzed
Normal skin	TS	3  (1, 8)	1	16 (exon 1 c-jun); 5 (exon 7 p53)
	NTS	10  (4, 16)	1	10 (exon 5 p53)
LF041 skin	TS	14  (4, 28)	6.1 ± 2.8	16 (exon 1 c-jun); 5 (exon 7 p53)
	NTS	28  (16, 32)	3.1 ± 1.1	10 (exon 5 p53)
LXSN lung	TS	3.5  (1, 8)	1	9 (c-jun promoter); 4 (exon 7 p53)
	NTS	6 (2, 16)	1	7 (exon 5 p53); 9 (exon 8 p53)
E6 lung	TS	12  (6, 24)	3.8 ± 1.4	9 (c-jun promoter); 4 (exon 7 p53)
	NTS	18  (8, 24)	4.1 ± 1.3	7 (exon 5 p53); 9 (exon 8 p53)
E7 lung	TS	4  (1, 8)	1.5 ± 0.8	9 (c-jun promoter); 4 (exon 7 p53)
	NTS	16  (4, 32)	3.6 ± 1.9	5 (exon 5 p53); 5 (exon 8 p53)
E6E7 lung	TS	13  (4, 28)	4.1 ± 0.9	9 (c-jun promoter); 4 (exon 7 p53)
	NTS	17  (6, 32)	4.1 ± 1.3	5 (exon 5 p53); 5 (exon 8 p53)

^*Each value reflects the average time required to achieve 50% repair, and was extrapolated from repair-rate determinations for at least 10 different dipyrimidine sites pooled from different gels. For example (as indicated by the first entry in the column at the far right), the average 50% repair time for the TS strand of normal skin fibroblasts was calculated by pooling repair-rate calculations from 21 sites, i.e., 16 from exon 1 of c-jun and 5 from exon 7 of p53. The values in parentheses represent the slowest and fastest 50% repair times, to indicate the site-to-site variation in repair rates.