Figure 4.

Both tlc1Δ and tel1Δ tlc1Δ cells form senescent colonies after 50 generations of growth. The top plate shows KR36-6L (wt), the tel1Δ and tlc1Δ haploid parents streaked for single colonies. The tlc1Δ strain had previously grown ≈50 generations without the TLC1 gene before streaking on this plate. The bottom plate shows a representative sample of four haploid progeny derived from a TEL1/tel1Δ TLC1/tlc1Δ diploid in which all strains had been grown for ≈50 generations before streaking on this plate.