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. 1999 Dec 21;96(26):15062–15067. doi: 10.1073/pnas.96.26.15062

Figure 4.

Figure 4

The number of NK cell/target cell immune synapses is unaffected by the presence of metabolic or cytoskeletal inhibitors. The first six rows show conjugates of YTS/KIR1 and 221/Cw6-GFP in the presence of 50 mM d-glucose (A), 50 mM azide (B), 13 μM antimycin-A (C), 50 mM 2-deoxyglucose (D), 50 mM 2-deoxyglucose (E), and 10 μM cytochalasin D (NK cell adopts a cone shape in the presence of cytochalasin D) (F). (G) KIR-Ig-coated beads attached to 221/Cw4-GFP. Each row, except E, shows the Nomarski image of a cell conjugate, the corresponding fluorescence, and an overlay of the fluorescence and Nomarski fields. E shows the Nomarski image of a cell conjugate, the corresponding fluorescence image, and the image of the cluster within the plane of the interface (in green box), demonstrating that the ring-like organization of HLA-C also is retained in the absence of ATP.