Figure 4.

Localization of DCs in wild-type (A) and c-kit⁻γ_c⁻ (B) thymi. Sections were analyzed by immunofluorescence for expression of the medullary marker MTS10 (13) (green) vs. the DC marker CD11c (red). An MTS10⁺ medullary zone [“M” (see arrow)] is visible (A). Many, but not all, of the thymic DCs are localized in the medullary area, and DCs are rarely found in the cortex (C). In B, MTS10⁺ epithelium fails to form medullary areas, and single or small clusters of CD11c⁺ thymic DCs are distributed throughout the thymus. (Bars = 30 μm.)