Figure 3.

Inhibitory effect of anti-p75/AIRM1 or anti-CD33 mAb on the in vitro proliferation of chronic myeloid leukemic cells. Cells were isolated on F/H density gradients from peripheral blood of four patients affected by chronic myeloid leukemias t(9;22) and analyzed for the surface expression of CD33, p75/AIRM1, and IRp60 by indirect immunofluorescence and FACS analysis (Left). Cells were cultured with GM-CSF either in the presence or in the absence of mAbs specific for p75/AIRM1, CD33, or IRP60 surface molecules. After 5 days, cells were harvested and viable cells were counted (Right); values indicate the percentages of viable cells recovered in each experimental condition vs. control cultures containing no mAb.