Skip to main content
. 1999 Dec 21;96(26):15097–15102. doi: 10.1073/pnas.96.26.15097

Figure 2.

Figure 2

Tissue-specific regulation of the GM-CSF enhancer. DNA fragments encompassing either the promoter alone (pGM), promoter plus the GM-CSF enhancer (pGM-GME), or promoter plus the SV40 enhancer (pGM-SV40E) were tested for enhancer function in cells transiently transfected with luciferase reporter gene plasmids. Luciferase activity was determined for both unstimulated (−) cells and after stimulation of the cells for 9 hr with 20 ng/ml PMA and 1 μM A23187 (2 μM A23187 for HUVEC) (+). Error bars represent the SEM of 2–11 transfections. All data points are expressed relative to stimulated pGM = 1. A schematic diagram showing the constructs used also is shown.