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. 1999 Dec 21;96(26):15115–15120. doi: 10.1073/pnas.96.26.15115

Figure 4.

Figure 4

Ectopic expression of PEG-3 in T98G and DU-145 tumor cells facilitates tumor formation in vivo in nude mice. (A) Ectopic expression of the rat PEG-3 gene in the low tumorigenic human glioblastoma multiforme cell line T98G induces a progressive tumorigenic phenotype. Pooled cultures of vector or rat PEG-3 cDNA-transfected Zeocin resistant T98G cells were injected into four athymic nude mice/group. Animals were followed weekly until palpable tumors were observed. No tumors developed in vector-transfected T98G cells by 5 mo at which time the experiment was terminated. (B) Ectopic expression of rat PEG-3 in DU-145 cells induces an aggressive oncogenic phenotype. DU-145 cells were infected with 100 plaque-forming units/cell of Ad.PEG-3 S or Ad.Vec (Null, replication incompetent Ad lacking the PEG-3 gene), and 48 hr later the cells were mixed with Matrigel and injected s.c. into athymic nude mice (four animals/group). Animals were monitored as above and tumor volume was determined after 1 mo. *, Statistically significant difference of P < 0.025.