Figure 2.

Reverse Transcriptase-PCR analysis of ERα and ERβ expression in w.t. and βERKO aortae. PCR was performed by using either ERα- or ERβ-specific primers on the product of an RT reaction with RNA derived from w.t. (W) or βERKO (K) aortae, or w.t. mouse uterus (U). The RT reaction was carried out in the absence (−) or presence (+) of reverse transcriptase. Primer pairs were selected to produce PCR products of 240 bp (ERβ) or 611 bp (ERα). The PCR products were then separated by agarose gel electrophoresis. A 100-bp DNA ladder (leftmost lane) is provided as a comparison for evaluating the size of the PCR products.