Figure 3.
Medial areas of uninjured and injured (Uninj and Inj, respectively) w.t. and βERKO mouse carotid arteries treated with vehicle or E2. (A–F) Representative segments from elastin-stained carotid artery sections are shown (×400). The medial area of each section is listed below. All area measurements were made on complete sections. (A) Wild-type uninjured (Uninj, 16 × 10−3 mm2). (B) Wild-type injured, vehicle-treated (Inj, −E2; 23 × 10−3 mm2). (C) Wild type injured, E2-treated (Inj, +E2; 17 × 10−3 mm2). (D) βERKO (Uninj; 16 × 10−3 mm2). (E) βERKO (Inj, −E2; 22 × 10−3 mm2). (F) βERKO (Inj, +E2; 15 × 10−3 mm2). (G) Summary of medial area data for all w.t. and βERKO mice (n = 30 and n = 27, respectively, for uninjured vessels, and n = 13–15 for each group of injured vessels). The medial areas did not differ between the w.t. and βERKO mice, either in the injured, vehicle-treated groups (Inj −E2; 20 ± 0.9 × 10−3 mm2 vs. 19.8 ± 0.6 × 10−3 mm2, respectively), or in the injured, E2-treated groups (Inj, +E2; 17.8 ± 0.6 10−3 mm2 vs. 16.8 ± 0.6 10−3 mm2, respectively). In G, all data are shown as mean ± SEM. *, P < 0.05, compared with both uninjured and injured estrogen-treated groups within the same genotype.