Skip to main content
. Author manuscript; available in PMC: 2008 Dec 20.
Published in final edited form as: Mol Cell. 2008 Jun 20;30(6):678–688. doi: 10.1016/j.molcel.2008.06.001

Figure 4. Phosphorylation Site Mutations in Bcl-2 Alter the Regulation of Autophagy.

Figure 4

(A) Representative images of GFP-LC3 staining during starvation in MC7.beclin 1 cells co-transfected with GFP-LC3 and indicated plasmid. Arrows denote representative cells containing GFP-LC3 dots (i.e. autophagosomal structures).

(B and C) Light microscopic quantitation of autophagy in MCF7.beclin 1 cells co-transfected with GFP-LC3 and plasmid indicated below x axis. In (B) plasmids expressed wild-type or indicated mutant cellular Bcl-2. In (C) plasmids express a chimeric viral/cellular Bcl-2 in which amino acids 24-34 of vBcl-2 are replaced with amino acids 25-95 from wild-type or indicated mutant cellular Bcl-2. Results shown represent mean ± SEM for triplicate samples of greater than 100 cells per sample. Similar results were obtained in three independent experiments.