Figure 4.

RT-PCR was performed on RNA purified from whole blood from the proband. The cDNA was amplified with specific BRCA2 primers. The sample was separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Two RT-PCR products (503 bp and 549 bp) were obtained from the patient (Lane 1). The sizes of the DNA marker are indicated to the left. The PCR products were cloned and sequence analysis revealed that the 549 bp band contained the inclusion of 46 bp of intron 21 (data not shown).