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. 2008 Aug 6;3(8):e2857. doi: 10.1371/journal.pone.0002857

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Grosskurth et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Double-label indirect immunofluorescence microscopy was performed on frozen sections of chicken (A–D), frog (E–H) and zebrafish (I–L) hearts with mouse monoclonal anti-β-catenin (C, G, K) and rabbit polyclonal U1013 anti-mXin (B, F, J) antibodies, and subsequently with a mixture of rhodamine-conjugated goat anti-rabbit IgG and fluorescein-conjugated goat anti-mouse IgG. Merged images (D, H, L) indicate co-localization of the two proteins. The differential interference-contrast (DIC) images correspond to the fluorescent images shown in respectively. Scale bar: 10 µm.