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. 2008 Aug 6;3(8):e2857. doi: 10.1371/journal.pone.0002857

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Grosskurth et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–F) Double-label indirect immunofluorescence was performed on frozen sections of zebrafish heart with monoclonal anti-troponin T or anti-plakoglobin (green, visualized by fluorescein-conjugated 2^nd antibody) and polyclonal anti-mXin (red, visualized by rhodamine-conjugated 2^nd antibody). Before mounting, the sections were treated with DAPI to stain nuclei (blue). Scale bar: 10 µm. (G–I) Double-label indirect immunofluorescence was performed on chicken heart section with monoclonal anti-N-cadherin (green) and polyclonal anti-mXin antibody (red). Scale bar: 10 µm.