Figure 2. RANTES and IP-10 conditioned CFP10-DCs induce pro-inflammatory in vivo primary immune responses to BCG.

For Panel A GM-CSF-DCs or CFP10-DCs were infected in vitro with 1 MOI BCG for 24h. DCs were extensively washed to remove extracellular bacteria. 5×10⁶ DCs were adoptively transferred into naïve mice. Seven days later the inguinal lymph nodes were removed and 5×10⁶ cells/ml were cultured in 10% RPMI1640 medium with 10% FCS for 48h. Levels of indicated cytokines were measured in culture supernatants. For B, prior to infection with BCG, GM-CSF-DCs were either untreated or incubated with neutralizing monoclonal antibody to RANTES or IP-10 for 4h, while CFP10-DCs were either untreated or incubated with 25 ng/ml RANTES or IP-10 for 12h. DCs were extensively washed and then processed as in Panel A. Data from one of three experiments are shown.