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. 2008 Aug 6;3(8):e2869. doi: 10.1371/journal.pone.0002869

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Salam et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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FLUO-3-AM labeled GM-CSF-DCs (profile a–c) or CFP10-DCs (profile d–f) were stimulated with 1 MOI BCG and real-time increase in intracellular calcium was monitored over a period of 5 min. For profiles b and c GM-CSF-DCs were incubated with 5 μg/ml neutralizing monoclonal antibody to RANTES and IP-10, respectively, for 2h, prior to BCG stimulation. For profiles e and f, CFP10-DCs were incubated with 5 ng/ml recombinant RANTES and IP-10, respectively, for 12h, prior to BCG stimulation. DCs were extensively washed prior to flow cytometry. Data from one of three experiments are shown.