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. 2008 Aug 6;3(8):e2869. doi: 10.1371/journal.pone.0002869

Figure 9. T cells co-cultured with IFN-γ or IL-12 conditioned CFP10-DCs mediate clearance of M. tb H37Rv from macrophages.

Figure 9

BCG infected CFP10-DCs (CFP10), conditioned or not with either 2 ng/ml IFN-γ (IFNg) or IL-12 or transformed with retroviruses expressing IFN-γ (retroIFNg) or IL-12p40 (retroIL-12), were co-cultured for 48h with BCG primed T cells. From this, T cells were enriched and cultured with M. tb H37Rv infected macrophages (Mph) for 48h. A separate group wherein macrophages conditioned with 2 ng/ml IFN-γ, prior to infection with M. tb H37Rv was also included as a control. Cells from all the groups were lysed and plated in serial dilutions on 7H11 agar plates for CFU monitoring 2–3 week later. Data are the mean±s.d. of three experiments. P<0.007 (Mph vs CFP10+IFNg), P<0.007 (Mph vs CFP10+IL-12); P<0.005 (Mph+IFNg vs CFP10+IFNg), P<0.005 (Mph+IFNg vs CFP10+IL-12); P<0.03 (CFP10 vs CFP10+IFNg), P<0.05 (CFP10 vs CFP10+IL-12).