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. 2008 Aug 6;3(8):e2869. doi: 10.1371/journal.pone.0002869

Figure 10. IFN-γ or IL-12 conditioned CFP10-DCs mediate clearance of M. tb infection in vivo in mice.

Figure 10

Groups of mice were infected with 1×106 M. tb H37Ra intravenously via the tail vein. Seven days post-infection, 10×106 unconditioned CFP10-DCs or CFP10-DCs treated with both IFN-γ and IL-12 together (2 ng/ml each) for 2h (conditioned CFP10-DCs) were injected into the tail vein of mice. A repeat injection with the same number of DCs was given 7 days following the first transfer. ‘Control’ represents mice infected with M. tb H37Ra followed by intravenous injection of PBS. Seven days following the 2nd transfer, mice were sacrificed and lung and spleen homogenates were plated onto 7H11 agar plates in serial dilutions for CFU monitoring. In parallel, infected mice were injected with anti-TB drugs as given in Materials and Methods . Data are the mean±s.d. of three experiments. For lungs, P<0.04 (Control vs Conditioned CFP10-DCs), P<0.03 (CFP10-DCs vs conditioned CFP10-DCs). For spleen, P<0.02 (Control vs Conditioned CFP10-DCs), P<0.02 (CFP10-DCs vs conditioned CFP10-DCs).