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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Cancer Res. 2008 Apr 1;68(7):2436–2446. doi: 10.1158/0008-5472.CAN-07-6822

Figure 1. T cells derived from wild-type FVB mice will induce apoptosis in MMC in vitro but fail to reject MMC in FVBN202 mice following AIT.

Figure 1

A) Flow cytometry analysis of MMC after 24 hrs culture with splenocytes of FVB mice following three color staining. Gated neu positive cells were analyzed for the detection of annexin V+ and PI+ apoptotic cells. Data are representative of quadruplicate experiments. B) Donor T cells were enriched from the spleen of FVB mice using nylon wool column following the rejection of MMC. FVBN202 mice (n=4) were injected with CYP followed by inoculation with MMC (4 × 106 cells/mouse), and tail vein injection of donor T cells. Control groups were challenged with MMC in the presence or absence of CYP treatment. Tumor growth was monitored twice weekly.