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. 1999 Dec 21;96(26):15155–15160. doi: 10.1073/pnas.96.26.15155

Figure 5.

Figure 5

(A) Molecular sieving chromatography of 500 pairs of L. longipalpis salivary glands on a TSK-2000SW column perfused with 10 mM Hepes, pH 7.0, and 0.15 M NaCl for 30 min, then a 10-min gradient to 1 M NaCl. Eluted fractions were tested for anticlotting activity on a human plasma recalcification time assay, shown in the symbols. (B) RP fractionation of the most active fractions of the size-exclusion chromatogram on a Hamilton PRP-3 column running a gradient from 10% to 60% acetonitrile in water plus 0.1% trifluoroacetic acid. Symbols indicate the plasma recalcification time with aliquots from the column after drying and reconstitution in 10 mM Hepes, pH 7.0/0.15 M NaCl. The sequence obtained by N-terminal Edman degradation is shown.