Table 1.
Full-length cDNA clones containing secretory leading sequences from L. longipalpis salivary glands
| Category clone | Size, bp | ORF* | Signal† peptide | Mature‡ protein, Da | Predicted pI§ | Sequence¶ similarity | Confirmation |
|---|---|---|---|---|---|---|---|
| Blood feeding | |||||||
| LuloAPY | 1,071 | 325 | 21/22 | 35,071 | 9.11 | Cimex apyrase (49/38) | pI, activity |
| Lulo5NUC | 1,839 | 572 | 25/26 | 60,628 | 6.94 | Mouse 5′-nucleotidase (54/46) | 5′-Nucleotidase activity |
| LuloHYA | 1,249 | 376 | 16/17 | 42,289 | 7.98 | Polistes hyaluronidase (46/38) | Hyaluronidase activity |
| LuloAC | 621 | 160 | 19/20 | 16,732 | 8.40 | Rattus selectin (45/37) | Anticlotting activity, Edman |
| LuloRGD | 519 | 76 | 23/24 | 6,160 | 3.77 | RGD motif (no matches) | ND |
| Sugar feeding | |||||||
| LuloAMY | 1,699 | 497 | 18/19 | 54,023 | 6.12 | Drosophila amylase (67/60) | α-Amylase activity |
| Unknown function | |||||||
| LuloAG5 | 905 | 271 | 19/20 | 28,886 | 8.85 | Drosophila antigen-5 (38/33) | ND |
| LuloYELLOW | 1,409 | 412 | 18/19 | 45,181 | 5.78 | Drosophila yellow protein (36/26) | ND |
| LuloSL1 | 713 | 139 | 20/21 | 13,929 | 8.59 | No significant matches | Edman, MS |
*
Number of amino acids of the translation products; †Signal peptide cleavage site predicted by the signalp (12) program; ‡Predicted molecular mass (Da); §pI of the putative mature proteins; ¶amino acid sequence similarities and identity (in parentheses) always representing a continuous sequence length longer than 75% of the predicted protein sequence, by using gcg-bestfit to nearest blast match of nonredundant National Center for Biotechnology Informaiton database, both using blosum-62 matrix. ND, not determined.