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. 1999 Dec 21;96(26):15178–15183. doi: 10.1073/pnas.96.26.15178

Figure 4.

Figure 4

Western blot analyses of fibrils and pili. (A) Western blot analysis of fibrils. The same amount of whole-cell lysate of myxo-filaments was loaded for each sample. Fibrils were detected by using mAb 2105. Shown is one major band of 66 kDa. Similar results were obtained with other minor bands that were also recognized by mAb2105, except for CDS, which did not contain one of the minor bands (24). (B) Western blot analysis of pili. Pili were sheared off myxo-filaments and detected with polyclonal antibody against PilA. Lanes 1–9: DK1622, DZ4148, SW504, LS300, DK10407, DK10405, DK1300, DK10409, and CDS. SW501 (same result as SW504), DK3470 (same as LS300), DK10416, and DK1253 (same as DK10405) are not shown.